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Pre-cultures of the strains to be tested were grown after overnight in 4 mL of growth in LB-K (37 C/300 rpm), and the next morning cells were diluted to 1:100 in either LB-K or MMA (pH = 7.4, 0.5% glycerol)

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aureus Since KL1s synergy probably depends on metabolism, we tested a mutant MRSA strain lacking all four glucose transporters (G4) to determine whether central metabolism was required 35

[DOI] [PMC free article] [PubMed] [Google Scholar] 253.Cletzer E., Klahn S., Dervisis N., LeRoith T

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