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All samples were dried in a Vacufuge Plus vacuum concentrator (Eppendorf) overnight

The peptide itself primarily interacts via its main chain with the lower lobe (especially with the five-stranded -barrel) and the domain motion is necessary to bring the scissile peptide group in proximity to the catalytic machinery of the enzyme

Myocardial scarring is thought to either induce or maintain ventricular arrhythmias in several ways: 1) scarring creates insulating barriers that form alterations in conduction in the fibrotic tissue, resulting in re-entrant wave fronts of excitation

Research protocols explore combination approaches with other senolytic compounds such as dasatinib and quercetin, providing frameworks for studying multi-target senolytic strategies and understanding synergistic effects in various experimental models and disease research applications