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After 180 min of anaerobic incubation at 37 C, the entire culture volume was collected into 50 mL conical tubes with pipetting and scraping to capture adherent cells, and the cells were centrifuged at 4000 g for 10 min, yielding pellets with a wet weight of ~100 mg

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Side Effects and Risks of Trigger Point Injection Therapy Touching to stabilize the affected muscle for injection is a common trigger point injection procedure