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Cells were washed twice with the FLICA kit wash buffer and then incubated with LIVE/DEAD Fixable AQUA Dead Cells Stain (Thermo Fisher, USA) for 15 min at RT, followed by extracellular staining in PBS + 1% BSA with the following fluorochrome-conjugated antibodies for monocyte phenotyping: anti-CD14 BV605 [Clone: M5E2], anti-CD16 PE-Cy7/BV711 [Clone: 3G8], anti-CD3 PE [Clone: HIT3a] and anti-CCR2 PerCP Cy5.5 [Clone: K036C2] are from BioLegend

10.1016/j.tibs.2009.08.005 Trends Biochem

The gut microbiota contributes to host metabolism and protects against pathogens, thereby affecting directly or indirectly host physiological functions (Pilla and Suchodolski, 2020)

(2020) have provided evidence that Brusatol, functioning as an Nrf2 inhibitor, demonstrated inhibitory effects in human NSCLC by instigating ferroptosis through modulation of the FOCAD-FAK signaling pathway