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Phosphate-buffered saline (PBS) at pH 7.4 is suitable for in vitro cell culture applications requiring a neutral, isotonic, buffered environment

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There is growing evidence, including findings obtained in experiments using heterochronic parabiosis in mice as a model system, that age-related changes in circulating anti-geronic factors importantly contribute to the regulation of vascular aging processes and the genesis of endothelial dysfunction and oxidative stress in a noncell autonomous manner [9]