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doi: 10.1016/0006-2952(88)90176-1

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To quantitate the mRNA expression levels of genes encoding transporters, heavy metal chelators, and antioxidative enzymes, the quantitative reverse transcription polymerase chain reaction (qRT-PCR) was conducted using a Rotor-Gene Q PCR cycler (Qiagen) and QuantiTect SYBR Green RT-PCR kit (Qiagen) as described previously (Kim and Kang, 2018)