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Assays were performed in 96 well plates and activity measured by monitoring the formation of DNP-GS at 340 nm ( = 9600 M- 1 cm -1 ) every 30 seconds for 10 min, in 100 mM potassium phosphate buffer (K 2 PO4) pH 6.8 at 25C [3]

The Pernicious Anemia Society notes that while there are several known symptoms of pernicious anemia, the disease affects each patient individually

[34] reported that when buthionine sulfoximine is used to inhibit the activity of GCS (which catalyzes the first step in GSH synthesis) a half life of 26 hours for cellular GSH is observed

These include spelt, wheat, rye, oats, and barley, all containing gluten, and rice, corn, and millet, which do not contain gluten

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