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LNCaP (primary prostate adenocarcinoma), 22Rv1 (castration-resistant prostate cancer), and H660 (neuroendocrine prostate cancer) cells were cultured in RPMI 1640 media supplemented with 10% FBS and 1% penicillinstreptomycin at 37 C with 5% CO2

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Studies utilizing electron paramagnetic resonance spectroscopy have quantified the SOD-like activity of GHK-Cu at approximately 1,200 units/mg, representing moderate antioxidant capacity relative to native SOD enzymes

Additionally, it produces paracrine signals that inhibit the release of pro-inflammatory mediators from M1, thereby promoting chondrocyte differentiation

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10.1038/s41580-022-00456-z 10.1038/s41580-022-00456-z [DOI] [PubMed] [Google Scholar] Simunkova M, Alwasel SH, Alhazza IM, Jomova K, Kollar V, Rusko M, Valko M (2019) Management of oxidative stress and other pathologies in Alzheimers disease