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Each well was then washed with a washing buffer solution provided by the company and 50 L of streptavidin-HRP solution was added to each well and the plate incubated at room temperature for 30 min with gentle shaking on a plate shaker

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Calves, Swine, and Sheep: 1 to 2 mL

doi: 10.1038/s41538-018-0011-y

New insights into the immunological effects of food bioactive peptides in animal models of intestinal inflammation

They might cause certain allergic reactions if not done properly. 2