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In addition to mitochondriaderived ROS, other intracellular sources also contribute to the oxidative milieu in PD

After 3 days, the media was collected, filtered, and then added to day 150 hMIDOs

For creatine dosage , the most researched protocol involves a loading phase of 20 grams daily (split into 4 doses) for 5-7 days, followed by a maintenance dose of 3-5 grams daily

32 A similar mechanism may be at play with ACR modification, as ACR modification of HDL inhibits SR-BI-mediated cholesterol efflux and macrophages become foam cells in the presence of ACR-HDL

Additionally, treatment with Tim-AIII significantly induced lipid ROS and iron accumulation, GSH depletion, lipid peroxidation, and decreased mitochondrial membrane potential, further indicating that ferroptosis was induced after treatment with Tim-AIII