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Data were acquired using a FACS sorter (BD FACS Aria III, Franklin Lakes, New Jersey, USA) and analyzed using FlowJo software (BD Biosciences, Franklin Lakes, New Jersey, USA)

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You mentioned tight junctions, and the way I think about tight junctions please correct me because Im going to get some or all of this wrong is that essentially they form like a cellular fence in the gut, and that transient opening or partial opening of these is a normal process