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Western blotting analysis EV protein was quantified by a BCA protein assay (Thermo Fisher Scientific)

Tumor burden is associated with increased expression of the GLUL mRNA and activity in the muscle of mammals and birds, implicating this enzyme as an evolutionarily conserved mediator of cachexia (Chen et al

Several studies have shown that soluble signaling molecules, in addition to gap junctions, are crucial in the transmission of bystander effects

In contrast, in many cancers, cyclin D-CDK4/6 kinases are hyperactivated and become independent of the mitogenic stimulation, thereby driving uncontrolled tumor cell proliferation

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PMID: 8877962 Heary, R.F., Albert, T.J., Ludwig, S.C., Vaccaro A.R.., Wolansky, L.J., Leddy, T.P., Schmidt, R.R.: Surgical Anatomy of the Vertebral Arteries