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This is in contrast with the generation of reactive oxygen species triggered by the standard radiation, at a lower dose but for a longer time than FLASH-RT, leading to the PPAR activation and consequent lipid peroxidation

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The reporter ion intensities were defined as intensities multiplied by injection time (to obtain the total signal) for each isobaric labeling channel summed over all MS/MS spectra matching to the protein group as previously validated (Tyanova et al., 2016)

The PVDF membrane was then incubated with an HRP-coupled secondary antibody (1:5000, China) for 1 h