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American Journal of Physiology, 274 (2), F315F327

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Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks

Suggested Usage: Take 1 capsule daily, or as directed by your healthcare practitioner

Smokers lose Vitamin C with every cigarette smoked, making them more susceptible to aging rapidly

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