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Bacteria were pelleted by centrifuging at 13000 x g for 3 mins and the supernatant was discarded

The thiolated poly (ethylene glycol) folate (SH-PEG 5000 -FA) was obtained by coupling SH-PEG-NH 2 (Mn 5,000) with Folate N-hydroxysuccinimidyl ester via a previously published method [29-31]

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They are also more pronounced at higher doses, providing another reason to start conservatively

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Taghzouti et al