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The phenotypes are not correlated with microenvironmental features, including proliferation rate, immune cell infiltration and vascularization, are retained when patient-derived cells are grown in vitro or as orthotopically implanted xenografts and are robust to changes in oxygen concentration, demonstrating their cell-intrinsic nature

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Cluster of orthologous groups (COG) analysis was performed using the Functional Analysis Tools of the T-REx pipeline

Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS