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Analysis of total proteome by tandem mass tag (TMT)-based quantitative proteomics Preparation of cell lysates and labelling of peptides with TMT isobaric mass tags was carried out using the commercially available kit and reagents (Thermo Fischer Scientific, #90110) according to the manufacturers protocol

Such a registry will provide information about the natural course of the disease and information about treatment interventions found to be effective could provide a basis for future clinical trials

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At the end of the third day and one hour after the last injection, cardiac puncture was made under anesthesia to obtain blood and gastric tissue samples for biochemical, molecular and histopathological analyses