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Science 281 , 416419 (1998)

It has long been recognized that ictal cerebral hypermetabolism is characteristic of seizure disorders, where increased cerebral oxygen (Meyer et al., 1966) and glucose consumption (Ingvar and Siesj, 1983) are observed during seizure activity in experimental epilepsy models and in humans (During et al., 1994)

10.1038/nn.2929 72 DinuzzoM.MangiaS.MaravigliaB.GioveF

doi:10.1080/07420528.2020.1784189

Tissues were then embedded in paraffin wax at 70C to facilitate sectioning

Approaches for investigating p K a values of individual Cys residues in proteins often require the use of other methods, as well, including spectroscopic detection of small differences between absorbances of thiolate and thiol around 240 nm [37, 38], NMR detection of shifting protonation state relative to solution pH [39], readouts associated with chemical reactivity [40, 41], or functional assays of the thiol/thiolate group of interest [42, 43]