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Clonal WT MEFs were generated in parallel from the same parental clonal lines, except using a plasmid expressing pSpCas9-2a-GFP and a non-targeting sgRNA (Supplementary Table 4)

Other effective molecules in terms of sperm motility and concentration are the H 2 S precursor SG1002 ( in vivo study on men, 750 mg of SG1002 daily) (78) and finally, the amino acid-derived N-thiocarboxyanhydrides (NTAs), which releases H 2 S in the presence of carbonic anhydrase, in vitro increasing progressive sperm motility and prolonging sperm survival through supported mitochondrial activity (59)

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Prevention of severe acute pancreatitis with cyclooxygenase-2 inhibitors: a randomized controlled clinical trial

10.1186/s40425-016-0152-y 12 KennedyL

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