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Cancer Cell 15 , 539550 (2009)

However, Bemis-Murcko scaffolds are defined only for connected compounds with ring systems, and 186 molecules (almost 20% of the ApisTox dataset) violate this condition (141 have multiple fragments, 45 do not have any rings)

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If you are working with a poor antibody or a very low-abundance protein, you could try to increase binding by incubating overnight

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