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The release of mtDNA, a pro-inflammatory DAMP, has been shown to activate innate immune responses [33]

Effects of muscarinic ACh receptors on heart failure and myocardial hypertrophy An increasing number of studies have shown that stimulating the muscarinic ACh receptor through drugs or neural transmission may become an important means of treating heart failure (77) (Fig

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[DOI] [PMC free article] [PubMed] [Google Scholar] 23.Usatyuk P.V., Fu P., Mohan V., Epshtein Y., Jacobson J.R., Gomez-Cambronero J., Wary K.K., Bindokas V., Dudek S.M., Salgia R., et al

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Then, cells were sedimented by centrifugation (14,000 rpm, 5 min) and resuspended in 1 mL of fresh 50 mM potassium phosphate buffer to measure the fluorescence using a Varioskanr TM LUX Multimode Microplate Reader (Thermo Fisher Scientific, Massachusetts, MA, United States)