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GST-tagged SIRT7 protein purification GST-SIRT7-WT, GST-SIRT7-T255A, GST-SIRT7-S259A, and GST-SIRT7-T255A/S259A in the bacterial expression plasmid pGEX-4T-3 were expressed in Escherichia coli BL21 following induction by 0.1 mM isopropyl -d-1-thiogalactopyranoside at 25 C

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