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Effect of 1 week of sleep restriction on testosterone levels in young healthy men. Jama 305.21 (2011): 2173-2174

Substitution of these critical leucine residues to tryptophan eliminated binding affinity, highlighting the importance of maintaining key hydrophobic contacts of leucine side chains, and not general hydrophobicity, which is further exemplified by the evolutionary conservation of leucine residues in LD motifs 41

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