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What Role Does TB-500 Play In The Glow Peptide Protocol

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Following vortexing and centrifugation at 16,000 g for 10 min at 4 C, the supernatant (70 l) was loaded into LCMS vials

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While GHSR1a constitutive activity is required for amplification of MC3R signaling, it has minimal effect on GHSR1a-MC3R dimer internalization, as treatment with the GHSR1a inverse agonist SP-analog only results in a small, nonsignificant reduction in internalization (Schellekens et al., 2013)