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Like iNap, cyto-SoNar and mito-SoNar located in cytosol or mitochondria of HAECs, respectively (Supplementary Fig

Western blotting analysis and enzyme-linked immunosorbent assay (ELISA) assay Whole liver lysates were prepared by sonication in HEPES lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 0.1% Tween 20, 10% glycerol, 0.1 mM Na orthovanadate, 1 mM NaF, 10 mM -glycerophosphate) containing protease inhibitor cocktail (EDTA-free Complete Mini, Roche, Meylan, France)

Yu B, Egbejimi A, Dharmat R et al (2018) Phagocytosed photoreceptor outer segments activate mTORC1 in the retinal pigment epithelium
Urbanska K, Orzechowski A
