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The supernatant was collected by centrifugation to detect the level of MDA

It contains two conserved peptide motifs, DSGIS and DSAPGS, which are recognized by -transducin repeat-containing protein (-TrCP)

In addition, for therapeutic application, it is preferable that an enzyme should have both structural and functional stabilities under severe conditions

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J Biol Chem 2001;276:48562-71