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Following normalization using the housekeeping gene, the 2Ct technique was used to calculate the fold changes in the expression levels, as described in 2.7 Detection of aflatoxin residues in eggs The egg was collected after the completion of the trial to detect the residual AFT using high-performance liquid chromatography (HPLC)

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An altered gene expression profile is further observed in aged astrocytes

At the intersection of methionine metabolism lies S-adenosyl-L-methionine (SAMe), a metabolite of extraordinary biological significance

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6 Department of Civil Engineering, Indian Institute of Technology (IIT) Delhi, India