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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3 UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3 UTR reporter plasmid (mut)

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Kessoku T, Sumida Y, Imajo K, et al

doi: 10.1074/jbc.272.44.27812

With the high-speed development of the economy, various types of batteries have been widely used in industries

The SLC25 family of proteins are mitochondrial carriers essential for the functioning of eukaryotes because they transport nutrients such as amino acids, carboxylic acids, fatty acids, cofactors, inorganic ions, and nucleotides across the mitochondria inner membrane for energy conversion and maintenance of the cell 34