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Cell-free glutathionylation experiments Fifty ng of recombinant IL-1sRII (R&D systems) or recombinant Lyn purified from HEK293T cells were treated with 100 mM of DTT for 20 min at room temperature to reduce free cysteines

Mice were infected intravenously through the tail vein at a dose of 510 5 C

2 10 6 cells from each sample were aliquoted for CyTOF staining

Following the Iso-seq protocol, circular consensus (CCS) of subreads was calculated, and subsequently classified as full-length or non-full-length based on the presence of SMART adaptors at both extremities

The phosphorylation of eNOS was also increased and peaked at 30 min after the BPC 157 stimulation