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In contrast, human dermal fibroblasts (hFBs) showed a more stable NAD+/NADH balance and limited senescence during in vitro expansion, highlighting a key metabolic distinction between stem cells and differentiated cells

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However, ongoing technological innovations, including single-cell sequencing, multiomics integration, CRISPR/Cas9 screening, and 3D organoid models, offer promising solutions to overcome these barriers (Table 2)

The table below provides a clear comparison of their key differences

Obecne ograniczenia bada obejmuj brak wielkoskalowych randomizowanych bada klinicznych u ludzi, wci niepene zrozumienie precyzyjnych mechanizmw molekularnych oraz brak szczegowych danych farmakokinetycznych u ludzi