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To verify the health status of the rats before and after administration, blood samples were collected at 0 and 24 h after administration of test substances and sent to the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University for blood tests to determine creatinine, alanine transaminase (ALT), and aspartate transaminase (AST) with normal range 0.20.7 mg/dL, 1356 U/L, and 34109 U/L, respectively