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Briefly, 500 bp upstream of the DNA targeted for removal, as well as the first three codons of the gene of interest (GOI) were PCR amplified with Upstream-Forward and Upstream-Reverse primers

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Application & Images Applications M00928-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee Recommend Dilution WB 1:2000 IHC 1:150 Flow Cytometry 1:100 Validation Images & Assay Conditions Click image to see more details HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSS (Right lane) cDNA for 48 hrs and lysed

The sample was centrifuged 18,000 g for 45 min before filtering the supernatant through 0.45 m syringe filter to remove any remaining cell debris

Millipore, Zug, Switzerland)