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Thirty to fifty micrograms of protein were separated on 8%, 10%, or 12% SDS-PAGE gels, transferred to 0.45 m nitrocellulose membranes (Thermo Scientific), and blocked with 5% nonfat dry milk in phosphate-buffered saline and 0.1% Tween 20 (PBS-T) or OneBlock Western-CL blocking buffer (Genesee Scientific

[DOI] [PMC free article] [PubMed] [Google Scholar] 69.Terruzzi I., Montesano A., Senesi P., Villa I., Ferraretto A., Bottani M., Vacante F., Spinello A., Bolamperti S., Luzi L

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