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Then 200 nM (200 l) of NIP-specific IgG1 variants were added to either the apical or basolateral reservoir and incubated at 37 C for 4 h before samples were taken from the opposite reservoir and analyzed by ELISA

If the inhibition percentage is high, the sample should be diluted properly

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In view of this, various strategies to improve peptide stability have been presented, including modifications of the C/N-terminus [34] and D -amino acid substitution [35]

1997;273:C1124C1129