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PMID 20498005

A large NIH-indexed observational study that followed 9,328 patients receiving outpatient IV treatments key IV therapy safety statistics that show only a small minority experienced side effects and nearly all of them were minor and short-lived

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The harvested cell pellet was resuspended in 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 mM imidazole and 0.05% 2-mercaptoethanol (lysis buffer) and lysed by sonication