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Statistical analysis Results were presented as mean SEM

Blood (10 mL) was collected from the participants in a serum tube without anticoagulant and left for 30 min at room temperature to confirm coagulation, followed by centrifugation at 2,000 g for 10 min

46 A randomized placebo-controlled trial in 29 postmenopausal women with fibromyalgia found 50 mcg of transdermal estradiol per day for eight weeks had no effect on pain and tenderness compared with placebo

Evaluation of outcomes with citalopram for depression using measurement-based care in STAR*D: Implications for clinical practice