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This strategy was effective, with all 24 evaluable patients showing engraftment, and only one case of hepatic SOS
The quantity of a target gene was calculated by normalization to GAPDH
The following day, Ni-NTA resin equilibrated in buffer A was added over the cleaved laccase samples and incubated at 25 C and 1,000 rpm in a thermomixer comfort shaker (Eppendorf) for 4 h
Between the thin air, the bustling pace, and long workdays, recovery matters
10.1016/j.alit.2015.07.003 61
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