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Materials and Methods Cell culture and transfections The HCT116 human colon carcinoma cell line and the derivative cell lines, HCT116 Pttg1 / , 4 HCT116 p53 / 33 and HCT116 p21 / , 34 were kindly provided by B Vogelstein (The John Hopkins Oncology Center)
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& Whitfield, A
