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EGF is bioactive but barrier-limited: the molecule does what it does only if it reaches the keratinocyte layer, and standard topical formulations deposit most of it on the stratum corneum where it has no relevant receptor

CrossRef Risk factors and economic burden of postoperative anastomotic leakage related events in patients who underwent surgeries for colorectal cancer Jeonghyun Kang, Hyesung Kim, HyeJin Park, Bora Lee, Kang Young Lee, Alberto Meyer PLOS ONE.2022

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thus, the JCV antibody assay achieved widespread use as the first blood-based biomarker test useful to stratify risk in MS