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Western Blot Analysis Each tissue sample (five individual samples per group) was homogenized in 10 volumes of lysis buffer (10 mM TrisHCl, pH 7.4, 1 mM EDTA, 2 mM Na 3 VO 4 , 1 mM phenylmethylsulfonyl fluoride, and 10 g/ml aprotinin) for 30 s using a polytron apparatus

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However, there was no significant protective effect from black tea [69]