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Research in Ligilactobacillus salivarius developed a new packaging method for probiotics, which means layer-by-layer (LbL) encapsulating a single bacterium with chitosan and alginate to tremendously increase the potential of Ligilactobacillus salivarius in alleviating colitis [107]

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Hepcidin binds to the only known cellular iron export protein, ferroportin1 (FPN1), leading to its internalization and degradation 23

A High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) was used to perform the reverse transcription following the manufacturers instructions and using the ProFlex PCR System machine (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA)

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