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To be labeled by U- 13 C 6 d-glucose, cells were washed once with warm PBS and replenished with fresh media lacking glucose (DMEM, no glucose, supplemented with 10% FBS and 1% GlutaMAX)
Biotinylated proteins were enriched by streptavidin pull down and analyzed by mass spectrometry (Fig
doi: 10.1074/jbc.R700045200
202 MoonD.-O.ParkS.-Y.LeeK.-J.HeoM.-S.KimK.-C.KimM.-O.et al (2007)
The appropriate secondary antibodies: goat anti-mouse IgG antibody (1:10000, Invitrogen, Cat
