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Indeed, all sperm selected for the study showed normal volume

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Thirdly, the lack of effective and specific drugs that can safely induce ferroptosis in cancer cells poses an additional challenge

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Reagents for cell culture and for SDS-PAGE and Western blot analyses were purchased from Gibco BRL (Grand Island, NY, USA) and from Bio Rad Laboratories (Richmond, CA, USA) respectively

Injection volume must also be carefully controlled, as animal studies indicate that reducing the volume can lower the risk of nucleus pulposus protrusion