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Finally, we also found the NO accumulating mutant nox1 to be highly sensitive to TMHT, as previously described at 45C (Lee et al., 2008) (Figure 4C)

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Nb(EgA1)- Strep -GG-Pap, expressed in NEBExpress(lowRF1) using a 100 mL culture and purified from the whole cell extract via -CD affinity chromatography, was dialyzed against HBST, and a dilution series (0.58 nM) was injected at a flow rate of 25 L/min

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