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To measure OCR in intact neonatal rat ventricular cardiomyocytes (NRC), NRC was seeded at a density of 8 10 4 cells per well into 0.1% w/v gelatin-coated Seahorse XFe24 plates with DMEM maintenance media containing 2% FBS with 1% antibioticantimycotic mixture (Gibco)

Room-temperature exposure for extended periods may reduce potency by 10-15% per month, depending on light exposure and humidity

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