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doi: 10.1590/1980-57642018dn13-030007

Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L

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After dialysis with Tris pH 8, the protein was purified and concentrated to 22 mg ml 1 (final buffer 50 mM Tris pH 9, 150 mM NaCl and 10% glycerol)
