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Following treatment, media was replaced with 80% (v/v) methanol (cooled to 80C) containing 500 nmol 6-chloronicotinamide (6-CN) as an internal standard (IS) to extract cellular metabolites

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[PubMed] [Google Scholar] 214.Das SK, Eder S, Schauer S, Diwoky C, Temmel H, Guertl B, et al