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The relative abundance of Erysipelatoclostridium was increased in vehicle-treated mice that underwent the WD to LD switch (WD/LD-V) relative to the two control groups (LD/LD-V and WD/WD-V) but was not increased in 5A-1MQ-treated mice that underwent the same diet switch (WD/LD-T)

this process involved denaturation, enzyme reaction, and thermal cycling to produce stable complementary DNA for subsequent analysis

Mice were initially group housed (three/cage) and allowed to acclimate to the colony environment maintained at a constant temperature (2123C) and humidity (4050%) on a 12-hour light-dark cycle (lights on 06001800 h)

Nature 2014

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Of 56 patients with limbic encephalitis attributed to VGKC antibodies in a laboratory, 49 had LGI1 antibodies and seven had Caspr2 antibodies