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Real-time PCR analysis Total RNA was isolated from cultured cells or kidney tissues using TRIzol (Invitrogen, #15596026), as previously described 34

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[DOI] [PMC free article] [PubMed] [Google Scholar] 33.Park S.H., Baek S.-I., Yun J., Lee S., Yoon D.Y., Jung J.-K., Jung S.-H., Hwang B.Y., Hong J.T., Han S.-B., et al
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Several proteins, such as caveolin-1 and hsp90, interact with and affect the overall activity of eNOS