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Cell viability assays The cells were plated in triplicate in 96-well plates (typically 7000 senescent and 2000 non-senescent cells)

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10.1093/jxb/erh253 25 EllouziH.HamedK.CelaJ.MllerM.AbdellyC.Munn-BoschS

Consistent with this notion, our supplementary experiment using a stronger SDS paradigm (5-minute defeat episodes) revealed a stress-intensity-dependent exacerbation of anxiety-like behaviors: WT mice, which showed no anxiety phenotype under the milder SDS condition, developed clear anxiety-like responses when the stress load was increased, whereas KI mice displayed even more pronounced behavioral impairments